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Attempted immunization of crossbred (Bos taurus x Bos indicus) calves by affinity purified concealed antigens of Hyalomma anatolicum anatolicum
G. Das, S. Ghosh, , M. Khan H., S. Gupta C.
Published in
Volume: 73
Issue: 7
Pages: 713 - 717
Concealed antigens from the adult of Hyalomma anatolicum anatolicum were isolated by immunoaffinity chromatography using anti-gut IgG as ligands. The bound antigens were characterized by SDS-PAGE and 2 proteins of 106.7 and 68 kDa were identified. Immunization of crossbred calves (Bos taurus x Bos indicus) with 1.7 mg of the bound antigens adjuvanted with FCA/IFA animal decreased 74. 4 and 52.2% population of nymphs and adults respectively. Besides, a significant percentages of abnormally fed larvae (P < 0.001) and nymphs (P < 0.05) was dropped from the immunized animals. Western blot analysis detected protein band of 68 kDa as the antigen responsible for the induction of resistance in the host.
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