Header menu link for other important links
Simulations Reveal Key Role of Arg15 for Promiscuous Activity in HisA Enzyme
, Warispreet Singh
Published in The Royal Society of Chemistry
The HisA enzyme catalyzes the first step of histidine biosynthesis via Amadori rearrangement of substrate ProFAR. Since it possesses the most conserved and ancient TIM-barrel fold{,} it provides an ideal framework for bioengineering a new function from ancestral enzymes. In the present study firstly{,} the catalytic mechanism of HisA biosynthesis was elucidated using hybrid Quantum Mechanical/Molecular Mechanical calculations{,} and thereafter{,} key residues contributing towards the promiscuity for TrpF activity were revealed using several MD simulations of wild type enzyme and its variant with the native (ProFAR) and promiscuous (PRA) substrates. Our study reveals that the two loops (βα)1 and (βα)5 on the catalytic site of the HisA enzyme{,} have incredible adaptability for the native and promiscuous substrates. The conformational interplay between these two loops is substrate driven and precise bioengineering targeting these loops is key to the emergence of new functions. Furthermore{,} the study reveals a key role of Arg 15 residue which is close to the catalytic center of the enzyme in the bifunctionality of HisA enzyme by increasing the loop flexibility. Therefore{,} our study provides crucial information for future bioengineering work to use the HisA enzyme as a scaffold for new enzymatic activity.
About the journal
Published in The Royal Society of Chemistry
Open Access
Impact factor