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Sample preparation, crystallization and structure solution of HisC from Mycobacterium tuberculosis
Nazia Nasir, , Bichitra Biswal K.
Published in
Volume: 69
Issue: 4
Pages: 445 - 448
Histidinolphosphate aminotransferase (HisC; Rv1600) from Mycobacterium tuberculosis was overexpressed in M. smegmatis and purified to homogeneity using nickel-nitrilotriacetic acid metal-affinity and gel-filtration chromatography. Diffraction-quality crystals suitable for X-ray analysis were grown by the hanging-drop vapour-diffusion technique using 30% polyethylene glycol monomethyl ether 2000 as the precipitant. The crystals belonged to the hexagonal space group P3221, with an unusual high solvent content of 74.5%. X-ray diffraction data were recorded to 3.08 {\AA} resolution from a single crystal using in-house Cu K radiation. The structure of HisC was solved by the molecular-replacement method using its Corynebacterium glutamicum counterpart as a search model. HisC is a dimer in the crystal as well as in solution. {\textcopyright} 2013 International Union of Crystallography.
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