Microbial load of nurses’ white coats in a hospital was determined on polyester cotton blend and polyester fabric after the first and second work shift. Contamination on the blend fabric was significantly higher (P < .05) than on polyester. After the second shift, bacterial colonies on polyester and blend fabrics increased by ~98\% and ~70\%, respectively. Among the isolates detected on polyester after the second shift, Escherichia coli was the most abundant (47.8\%), followed by staphylococci (19.1\%). © 2017 Association for Professionals in Infection Control and Epidemiology, Inc.