The anatomy of letterforms defines the structural formation of letters. The study is based on semiotic approach. The methods used here are Syntagmatic and Paradigmatic analysis. The anatomy is developed through analysis based on the work on Latin letterforms from three different aspect which are structural grid lines, anatomical features and parameters. This syntagmatic analysis is yielded in identification of various structural features of letterforms like terminal, bowl, blob, stem, dot or nukta, ascender and descender. The analysis has been carried out using two techniques, repeated forms and unique forms of letters. The paradigmatic analysis discusses the comparative study of structure and feature of letterforms across different typefaces such as Lohit Bengali, Vrinda, Solaimanlipi and etc. The analysis offers distinct anatomical nomenclatures after analyzing paradigmatic transformations. Further the study categorizes the letterforms according to the appearance of common features. © Springer India 2015.