Determination of maintainer and restorer status of germplasm collections is a prerequisite for their use in development of hybrid employing CMS system. In the present study, restorer and maintainer status of 372 Indian genotypes was determined using fertility restoration (Rf) linked microsatellite marker RM6100. A total of seven alleles for the marker locus, including four novel and three known, were identified in the study material. Maximum frequency was observed for the 144bp allele (44.89%) while minimum frequency was for 138bp allele (0.27%).Though most of the novel alleles were minor, occurring in less than 5% frequency, cumulatively they accounted for 9.2% frequency in the study material. Of the known alleles, which were found in 90.8% (338 genotypes) of the tested rice genotypes, 36% had maintainer type allele (132bp allele similar to Pusa 6B) while the rest had restorer type (144 or 150 bp) alleles. The spikelet fertility data generated on a subset of the study material agreed well with the established associations for the already reported alleles. Of the novel alleles, two (141 and 147 bp) were represented in the spikelet fertility dataset, both of which restored fertility, though their representation was low. This allelic information would be useful in selection of parental lines for development of hybrids in rice. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.