A second subterranean species of Pangio is described from an old dug-out well in Kerala, Southern India. The new species, Pangio pathala is unique within the genus in possessing the highest number (27) of caudal vertebrae. Pangio pathala is distinguished from P. bhujia, the only subterranean Pangio species known so far, in having four pectoral-fin rays (vs. three), five anal-fin rays (vs. six), 67 vertebrae (40 abdominal and 27 caudal vertebrae) (vs. 62–63), and a raw genetic distance of 8.1–8.7% in the mitochondrial cytochrome oxidase subunit 1 gene. This paper also provides an additional record of Pangio bhujia from a location 40 km south of the type locality. Copyright © 2022 Magnolia Press.