This work is an extension part of the Assembly Line Worker Assignment Balancing Problem(ALWABP), aims to provide a dynamic solution for an assembly line fatigue worker job rotation by using machine learning based digital twin. This framework explains, fatigue worker identification and work rotation possibilities for a reconfigurable assembly line. The fatigue causing parameters are sensed from the workers and classified with a fatigue classifier then send the fatigue worker details to a worker job rotation search algorithm. The job rotation search algorithm provides a suggestion to the production supervisor for a best possible worker job rotation/reallocation solution dynamically. © 2021 The Author(s).